Development of a microplate reader compatible microfluidic device for enzyme assay
نویسندگان
چکیده
We report a novel platform technology of enzyme assay using microfluidic channel. Employing the patterns of microfluidic channel on a plastic chip, the dilution of substrate solution and enzyme reactions are sequentially carried out in microfluidic channels. The microfluidic device is fabricated by micromolding processes with polydimethylsiloxane (PDMS) polymer. The device consists of microfluidic channel modules running four assay tests on a single device and each module comprises six reaction chambers linked with microfluidic channels. Overall dimension of the microfluidic device is the same size as a 96-well microtiter plate and 24 reaction chambers in device adapt to the c a e c i ( ©
منابع مشابه
Dihydrofolate reductase enzyme inhibition assay for plasma methotrexate determination using a 96-well microplate reader.
Microplate reader assays offer several advantages over conventional spectrophotometric assays. We adapted the dihydrofolate reductase (DHFR) enzyme inhibition assay for use in a 96-well microplate reader to measure plasma methotrexate (MTX) concentrations. The assay is linear from 0.01 to 0.1 micromol/L. The within-run CVs at 0.03 micromol/L and 0.08 micromol/L MTX were 4.0% and 2.7%, respectiv...
متن کاملA paper/polymer hybrid microfluidic microplate for rapid quantitative detection of multiple disease biomarkers
Enzyme linked immunosorbent assay (ELISA) is one of the most widely used laboratory disease diagnosis methods. However, performing ELISA in low-resource settings is limited by long incubation time, large volumes of precious reagents, and well-equipped laboratories. Herein, we developed a simple, miniaturized paper/PMMA (poly(methyl methacrylate)) hybrid microfluidic microplate for low-cost, hig...
متن کاملRapid Measurement of Chlorophylls with a Microplate Reader
This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. The procedure enables direct translation of any published equation developed on a 1-cm pathlength spectrophotometer to any format microplate with any sample volume. Chlorophylls were extracted with methanol because it is compatible with cheap polystyrene plates; however, the principles apply t...
متن کاملLuciferase Measurements using the ClarityTM Luminescence Microplate Reader
Reporter genes, such as luciferase, are important tools for studying gene expression. The use of reporter genes allows the in vitro and in vivo measurement of gene expression from virtually any endogenous genetic control element. Luciferase enzyme and its subsequent luminescent reaction is often the gene reporter of choice for many experimental conditions. Here we describe the measurement of lu...
متن کاملQuantitative Beutler test for newborn mass screening of galactosemia using a fluorometric microplate reader.
BACKGROUND The Beutler enzyme spot test is an effective assay for newborn mass screening of galactosemia, but it is qualitative and relies on visual interpretation. We describe a quantitative, instrumental modification of the assay. METHODS We modified the macroscopic visual Beutler enzyme spot test by adding extraction of blood components from filter paper, deproteinization with acetone-meth...
متن کامل